Table 2.
Intensity of C3 deposition in kidneys of mice with glomerulonephritis and controls.
| Mouse | Intensity of C3 staining |
|||||
|---|---|---|---|---|---|---|
| Total number of glomeruli | 0 (%) | 1 (%) | 2 (%) | 3 (%) | Totala | |
| Untreated | ||||||
| 1 | 48 | 8 (16·7) | 23 (47·9) | 9 (18·8) | 8 (16·7) | 65 |
| 2 | 46 | 21 (45·7) | 11 (23·9) | 4 (8·7) | 10 (21·7) | 49 |
| 3 | 51 | 5 (9·8) | 21 (41·2) | 14 (27·5) | 11 (21·6) | 82 |
| Treated with the B1R antagonist | ||||||
| 4 | 56 | 38 (67·9) | 7 (30·4) | 1 (1·8) | 1 (1·8) | 22 |
| 5 | 55 | 31 (56·4) | 15 (27·3) | 7 (12·7) | 2 (3·6) | 35 |
| 6 | 42 | 17 (40·5) | 22 (52·4) | 0 (0) | 3 (7·1) | 31 |
| 7 | 47 | 25 (53·2) | 15 (31·9) | 4 (8·5) | 3 (6·4) | 32 |
| 8 | 50 | 30 (60) | 13 (26) | 7 (14) | 0 (0) | 27 |
| Controls | ||||||
| 9 | 39 | 34 (87·2) | 4 (10·3) | 1 (2·6) | 0 (0) | 6 |
| 10 | 42 | 28 (66·7) | 15 (35·7) | 4 (9·5) | 0 (0) | 23 |
| 11 | 46 | 37 (80·4) | 7 (15·2) | 2 (4·3) | 0 (0) | 11 |
| 12 | 51 | 38 (74·5) | 10 (19·6) | 3 (5·9) | 0 (0) | 16 |
a
All glomeruli in each section were counted and graded in blinded fashion after intensity as follows: no staining (0), low (1+), medium (2+) and high (3+) as depicted in Supplementary Fig. S1. The total level of intensity was calculated for each section as the number of glomeruli multiplied by the level of intensity.