Fig. 2.

Nwd1 location in excitatory synapses acute seizures brain tissues.

(a–b) Immunofluorescence staining for Nwd1 in the cerebral cortex of individuals with TLE patients. Nwd1 co-localizes with excitatory synapses marker postsynaptic density protein PSD95 but presynaptic vesicular marker Vglut1 (a) and less in inhibitory synapses marker GAD67 (b). (c–d) Immunofluorescence of Nwd1 in the hippocampi from KA-induced acute seizures mice show that Nwd1 colocalizes with PSD95 and lacks of colocalization of Nwd1with Vglut1 (c) and less in GAD67 (d) in the neuronal cells.