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. 2019 Aug 29;47:470–483. doi: 10.1016/j.ebiom.2019.08.050

Fig. 3.

Fig. 3

Downregulation of Nwd1 weakens glutamatergic synaptic transmission in hippocampal pyramidal neurons.

(a) Representative mEPSC recordings (left) and average waveforms (right) for the control (Con), AAV-GFP-injected and AAV-Nwd1-shRNA-injected mice. (b) The quantitative analysis of mEPSC frequency and amplitude (n = 14 neurons for Con, n = 13 neurons for AAV-GFP; n = 15 neurons for AAV-Nwd1-shRNA). (c) Representative mIPSC recordings (left) and average waveforms (right) for the mice in all groups. (d) The quantitative analysis of mIPSC frequency and amplitude (n = 12 neurons for Con, n = 13 neurons for AAV-GFP; n = 11 neurons for AAV-Nwd1-shRNA). (e) Representative images of the dendrites of primary hippocampal neurons treated with either AAV-GFP, AAV-Nwd1-Scr or AAV-Nwd1-shRNA. (f) The summary of spine density (n = 12 neurons for AAV-GFP, n = 11 neurons for AAV-Nwd1-Scr; n = 12 neurons for AAV-Nwd1-shRNA). Scale bars: upper panels, 25 μm; lower panels, 10 μm. The error bars indicate the SEM. ***P < 0.001, P ≥ .05 was considered not significant (N.S.). One-way analysis of variance (ANOVA), followed by Tukey's test.