Fig. 5.

Phosphorylation of Tyr1472 in GluN2B is suppressed in the hippocampal neurons after decreasing Nwd1 activity.

(a) Representative western blots for GluN2A, GluN2B, p-Tyr1472 and p-S1480 in the primary hippocampal neuron lysate obtained from AAV-Nwd1-shRNA, AAV-GFP and control neurons. (b) Quantitative analysis of data in (a). The surface protein amount of GluN2B, but not of GluN2A, is decreased after AAV-Nwd1-shRNA treated, and p-Tyr1472 intensity is decreased. However, p-Tyr1472 intensity did not change after application of inhibitor PP2 to the AAV-Nwd1-shRNA treated groups. Phosphorylation of GluN2B at S1480 showed no change in the neurons treated with AAV-Nwd1-shRNA compared to control and AAV-GFP group (n = 6 mice for each group). Error bars indicate SEM. ANOVA followed by Tukey's test. **P < 0.01, ***P < 0.001. N.S., not significant.