Table 1.
Panel of 10 microsatellites used in the genetic analysis of T. circumcincta populations. The optimal annealing temperature (TA) for each primer pair is listed along with the size range of the amplified alleles. Primer sequences are those reported byaGrillo et al. (2006) andbRedman et al. (2015).
| Microsatellite | Repeat Sequence | TA (°C) | Size range of alleles (bp) | Primer sequences (5′ → 3′) (Sense (S) and Antisense (A)) |
|---|---|---|---|---|
| MTG 15a | (GT)6GC (GT)6GGGTTTGT | 58 | 233–275 | S: TGCAAGGAAACTGCTAAGAAGGAG A: ATCATGGAACCTTGATACCGCAAG |
| MTG67a | (CT)14 | 58 | 172–192 | S: CAAGTCGTTTAGGCACGTCTGG A: CAGGGCGGAACCCAATTGATCG |
| MTG68a | (ACA)2 (ACC)2TCG (ACA)4ACTACAACCA CAACTACG (ACAACC)2 |
50 | 420–453 | S: ATCACCAGGCGGCTGCTACG A: CGAAAAGTAGAGTATGAGC |
| MTG73a | (TGC)2TGTTGC (TGT)2 (TGA)3TG (TGA)2T AA (TGA)2AAATA (TGA)2 |
45 | 148–157 | S: CCTTGTATAAATTCGAAGC A: GTAGTAGTGATTAACTTCCG |
| Tc 2066b | [GGCGAGTA]9GGCGATTAGGCGAGAA GGCGTGTAGGTGAGTAGGCGAG |
50 | 189–309 | S: GAGCAACGACTGAACCTCAC A: GCTGGAAGCATATTCTGCGC |
| Tc2467b | [TTTA]15TCTATT | 54 | 149–224 | S: AACGCTTTGAACCGTGTCGG A: GCTGCCACATCAGCTTAGA |
| Tc4504b | [ACAT]13ACAGATACAGACA | 50 | 228–276 | S: TTATCACACCACTTCATT A: GTCTTTAAACGCTAAATA |
| Tc7989b | [GTCT]17GTCCGTTTGTGTG | 50 | 133–232 | S: GATCTCACGTACTATGAA A: CTATTGAATGTCGTACAG |
| Tc13604b | [CAGGTA]12TAGGTACAGGCACAG | 50 | 250–386 | S: CGATAAATGGTATTATCTG A: GCTGCTATTAGAGGATAT |
| Tc22274b | [TGTA]17TGTCTGAATGT | 54 | 189–329 | S: ACAAAGTGCTCAAGTTAG A: GGGGGTTCTATATACAGTA |