Fig. 5.

Animal distress before and during tumour growth. Distress score (A), burrowing behaviour (B), percentage of body weight change (C), nesting score (D), HR (E), Act (F), BT (G) and FCMs (H) were evaluated before (−1) carcinoma cell injection (CI) and on days 4, 18, 34 after cancer cell injection. Significant differences of non-parametric data and data in percent were evaluated with the Friedman test followed by Dunn’s method (A-E). Parametric data was determined by repeated measures one-way ANOVA followed by pairwise comparison via Tukey’s test (F–H): P ≤ 0.05 significant to indicated day (n = 7). For details of the data, see Table 4.