Fig. 8.

Performance of each parameter to quantify distress after carcinoma cell injection. To evaluate the performance of each parameter, the area under the curve (AUC), the 95% confidence interval (CI) and the P-value (p) was assessed by performing receiver operating characteristic curve (ROC) analysis on data from mice before (−1) and after tumour cell injection (0). The distress score (A), burrowing behaviour (B), body weight change (BW change) (C), nesting behaviour (D), HR (E), Act (F), BT (G) and FCMs (H) were evaluated (n = 9).