Figure 3.
Knockdown of circARHGAP10 Suppresses the Proliferation of NSCLC Cells by Downregulating GLUT1
(A) qRT-PCR detection of circARHGAP10 expression following transfection with siRNA against circARHGAP10 (sicircARHGAP10) or the negative control (NC). Data are presented as the mean ± SD. ***p < 0.001 versus NC. (B and C) Both 18F-FDG uptake (B) and lactate production (C) were decreased in the cells after silencing circARHGAP10. (D) qRT-PCR detection results revealed that GLUT1 expression was significantly downregulated after circARHGAP10 silencing in both A549 and H1650 cells. (E and F) CCK8 assays were used to evaluate cellular proliferation in both (E) A549 and (F) H1650 cells. Data are presented as the mean ± SD. ***p < 0.001 versus NC. (G and H) Cloning formation assay showing the level of cellular proliferation in both A549 and H1650 cells (G). The relative cloning number was calculated (H). Data are presented as the mean ± SD. ***p < 0.001 versus NC. (I) Representative photographs of A549 tumor formation in the xenografts of nude mice. (J) Summary of the tumor volume in mice that were measured weekly. Data are presented as the mean ± SD. ***p < 0.001 versus NC. (K) Tumor weight was measured 30 days post-injection. Data are presented as the mean ± SD. ***p < 0.001 versus NC. (L) Immunohistochemical analysis shows the percentage of Ki-67-positive cells in the xenograft tumor tissues. (M) Immunohistochemical analysis shows the expression of GLUT1 in the tumor tissues.