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. 2019 Aug 21;18:219–231. doi: 10.1016/j.omtn.2019.08.016

Figure 6.

Figure 6

GLUT1 Overexpression Reverses the Inhibitory Effect of miR-150-5p on Cellular Proliferation and Metastasis In Vitro

(A) The predicted binding sites of miR-150-5p with the 3′ UTR of GLUT1. The mutated version of 3′-UTR-GLUT1 is also shown. (B) The relative luciferase activity was determined 48 h after transfection with miR-150-5p mimic/normal control or with the 3′-UTR-GLUT1 wild- or Mut-type in 293T cells. Data are presented as the mean ± SD. ***p < 0.001. (C and D) qRT-PCR detection showing the expression of miR-150-5p in both (C) A549 and (D) H1650 cells. (E and F) qRT-PCR detection showing the expression of GLUT1 in both A549 (E) and H1650 (F) cells. Data are presented as the mean ± SD. ***p < 0.001 versus NC. (G and H) CCK8 assay showing that GLUT1 overexpression rescued the proliferation of both (G) A549 and (H) H1650 cells after the upregulation of miR-150-5p. Data are presented as the mean ± SD. ***p < 0.001 versus NC. ###p < 0.001 versus mimic. (I and J) Cloning formation assays showing that the overexpression of GLUT1 rescued the proliferation of both A549 and H1650 cells following the upregulation of miR-150-5p (I). The relative cloning numbers were calculated (J). Data are presented as the mean ± SD. ***p < 0.001 versus NC. ###p < 0.001 versus mimic. (K–M) Wound-healing assays showing that the overexpression of GLUT1 rescued the invasion of both A549 (K and L) and H1650 (K and M) cells following the upregulation of miR-150-5p. Data are presented as the mean ± SD. ***p < 0.001 versus NC. ###p < 0.001 versus mimic. (N–Π) Transwell assay showing that GLUT1 overexpression rescued the migration of both A549 (N and O) and H1650 (N and P) cells following the upregulation of miR-150-5p. Data are presented as the mean ± SD. ***p < 0.001 versus NC. ###p < 0.001 versus mimic.