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. 2019 Oct 9;25:7581–7590. doi: 10.12659/MSM.916522

Figure 5.

Figure 5

HDAC4 takes part in regulating proliferation of VSMC. (A) The expression of HDAC4 in PDGF-bb-stimulated VSMCs was measured by qRT-PCR. (B–D). qRT-PCR and Western blot analysis were were conducted to detect the overexpression efficiency of pcDNA3.1-HDAC4. (E) The proliferation of VSMCs transfected with pcDNA3.1-HDAC4, empty vector, or control were detected by MTT assay. (F–H) The mRNA and protein level of PCNA in VSMCs transfected with miR-149-5p mimics, inhibitor, or control. * p<0.05 and ** p<0.01 vs. control.