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. 2019 Aug 14;18:88–98. doi: 10.1016/j.omtn.2019.07.023

Figure 4.

Figure 4

miR-197-3p Decreases the Expression of the Tumor Suppressor Gene FBXW7

(A) Predicted binding sites of miR-197-3p in the 3′ UTR of FBXW7 mRNA according to TargetScan. (B) The relative expression level of FBXW7 in breast cancer cell lines. (C and D) Luciferase reporter assay of BT549 (C) and 4T1 (D) cells cotransfected with miR-197-3p mimics and the FBXW7 3′ UTR wild-type or mutant luciferase reporter. (E) The expression of FBXW7 was decreased after transfection with miR-197-3p mimics. (F) The expression of FBXW7 was increased after transfection with miR-197-3p inhibitors. (G and H) Kaplan-Meier analysis of the (G) overall survival and (H) disease-free survival of 473 TNBC patients with high (green) or low (blue) FBXW7 expression levels. *p < 0.05, **p < 0.01. SD is shown as error bars.