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. 2019 Sep 12;18:308–319. doi: 10.1016/j.omtn.2019.09.002

Figure 3.

Figure 3

Cellular Uptake of miR-885-5p in Hepatoma Cells and Release of miR-885-5p

(A) Cellular uptake of miR-885-5p-L (liposome) in SMMC-7721 and Huh7 cells visualized by fluorescence microscope. Hepatoma cells were incubated with cy3-labeled miR-885-5p-L (100 nM miR-885-5p) for 1.5 h and then stained with DAPI and observed under a fluorescence microscope (200×). (B) Cellular uptake of miR-885-5p in SMMC-7721 and Huh7 cells determined by flow cytometry analysis. SMMC-7721 and Huh7 cells were treated with miR-885-5p-L (100 nM miR-885-5p). (C) The expression levels of mature miR-885-5p in hepatoma cells were detected by quantitative real-time PCR. All data are presented as mean ± SEM of 3 independent experiments. ***p < 0.001; ****p < 0.0001.