Figure 4.
miR-885-5p Negatively Regulated the Malignant Phenotypes of HCC In Vitro
The cells transfected with miR-NC and miR-885-5p under normoxia or hypoxia conditions induced by CoCl2 and DFX were subjected to CCK8 analysis, wound healing assay, cell migration, and apoptosis assay. (A) Effects of miR-885-5p on proliferation over different time periods of Huh7 and SMMC-7721 cells. (B) Images of cell migration from wound healing assay. Scratch wounds were made on confluent monolayer cultures 48 h post-transfection. Images of wound repair were taken at 0, 24, and 48 h after wound (40×). The percentage of wound closure was normalized to the wound area at hour 0. (C) Transwell assay of SMMC-7721 cells treated with miR-NC or miR-885-5p (200×). Histograms indicate the relative percentage of cells across the membrane. The relative percentage of migrating cells from control group is designated as 100%. (D) miR-885-5p induced cell apoptosis. Cell apoptosis was detected by annexin-V/propidium iodide combined-labeling flow cytometry in SMMC-7721 cells 48 h after transfection. Quadrants from lower left to upper left (counterclockwise) represent healthy, early apoptotic, late apoptotic, and necrotic cells, respectively. Apoptotic evaluation was determined by the percentage of apoptotic cell number in total cell number. All data are presented as mean ± SEM of 3 independent experiments. The comparisons were evaluated using t test. **p < 0.01; ***p < 0.001; ****p < 0.0001.