Figure 5.

miR-885-5p Inhibited Tumorigenicity of HCC In Vivo

(A) Xenograft tumors in nude mice. (a) Morphology of tumors excised from mice at the end of experiment. (b) Growth curves of xenograft tumors. Overexpression of miR-885-5p reduced the volume of the xenograft tumors. (c) The body weight of mice after sacrifice. The results are displayed as mean ± SEM of 5 animals per group. (B) Ki-67 staining in biopsies (200×). For each treatment, 3 independent fields from different biopsies were counted. (C) Immunohistochemical analysis of apoptosis proteins Bcl-2, Bax, and Caspase-3 (200×). (D) miR-885-5p suppressed the expression of HIF-1ɑ, GLUT1, HK2, and LDHA in xenograft microenvironments. The immunohistochemical analysis from excised tumors of Warburg-effect-related enzymes was evaluated. Protein signal was stained by DAB (brown), and cell nucleus was stained by DAPI (blue). **p < 0.01; ***p < 0.001.