Figure 3.

Overexpression of miR-143 Affects Proliferation, Migration, Invasion, and Apoptosis in Prostate Cancer Cells

(A) The effect of overexpressed miR-143 on the proliferation of PC-3 cells determined by EdU assay (scale bar, 50 μm). (B) The apoptosis rate of PC-3 cells with overexpressed miR-143 by flow cytometry. (C) The effect of overexpressed miR-143 on the migration of PC-3 detected by Transwell assay (scale bar, 50 μm). (D) The effect of overexpressed miR-143 on the invasion of PC-3 detected by Transwell assay (scale bar, 50 μm). (E) The expression of miR-143 after overexpressing miR-143 in PC-3, measured by qRT-PCR. (F) The effect of overexpressed miR-143 on the expression of TFF3, determined by qRT-PCR and western blot analysis. *p < 0.05 compared with the corresponding mimic-NC group; the measurement data were expressed using mean ± SD; statistical analysis was performed using an independent sample t test, and the experiment was repeated three times. PC-3, prostate cancer cell line-3; EdU, 5-ethynyl-2′-deoxyuridine; TFF3, trefoil factor 3; NC, negative control.