Fig. 8.

AUNIP controls cell cycle progression in vitro and its expression is associated with poor survival in OSCC patients.

a. AUNIP mRNA levels in indicated cells transfected with AUNIP siRNA. b. AUNIP protein levels in indicated cells transfected with AUNIP siRNA. c. Representative images (left) and quantification (right) of Blank, Negative Control (NC) siRNA-, and AUNIP siRNA- transfected SCC-9 and SCC-15 cells were analyzed in a colony formation assay. d. Representative images (left) and quantification (right) of Blank, Negative Control (NC) siRNA-, and AUNIP siRNA- transfected SCC-9 and SCC-15 cells were analyzed in cell cycle assay. e, f. Kaplan-Meier analysis of overall survival was performed to indicate higher expression of AUNIP was correlated with poor survival of OSCC patients in GSE41613 and TCGA datasets. P-values were obtained from the log-rank test. n.s, not significant, *P ≤ 0·05, **P ≤ 0·01, ***P ≤ 0·001, and ****P ≤ 0·0001 were obtained by Student's t-test. All data are represented by mean ± SD.