Skip to main content
. 2019 Oct 10;7:206. doi: 10.3389/fcell.2019.00206

Figure 5.

Figure 5

Individual dAkt isoforms facilitate dInR induced cellular insulin signaling. (A) Protein samples from flies kept on normal food (NF), and food from stationary (SP), or exponentially (EP) grown S. cerevisiae (S) or C. oligophagum (C). Shown is a photograph from a Western-Blot membrane of adult head-samples from flies taken 7 days after transfer from normal food (NF) to the respective yeast diet probed for mCherry (mCherry-FOXO), phosphorylated dAkt (AktThr342 or AktSer505), pan-dAkt protein (pan Akt), and Tubulin (Tub). (B–D) Quantification of three independent Western-Blot replica from head-samples (9 heads per sample) of adult flies taken 7 days after transfer from normal food (NF) to the respective yeast diet (SSP, SEP, CSP, or CEP). Shown are relative ratios of pan-dAkt85/Tubulin (B), phosphorylated dAkt85−Thr342/pan-dAkt85 (C), and dAkt85−Ser505/pan-dAkt85 (D).