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. 2019 Oct 10;10:2294. doi: 10.3389/fmicb.2019.02294

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Copyright © 2019 Patel and Free.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Structures of N. crassa N-linked oligosaccharides as determined by glycan profiling experiments. The N-linked oligosaccharide (left) was isolated from cell wall glycoproteins synthesized by the Δoch-1 mutant, which is unable to elaborate the galactomannan. The full length galacatomannan (middle) was isolated from cell wall glycoproteins synthesized by the Δdfg-5,Δdcw-1 mutant, which lacks the α-1,6-mannanases needed to process the galactomannan. The processed galactomannan (right) was isolated from cell wall glycoproteins synthesized by the Δgel-1,Δgel-2,Δgel-5 mutant, which is unable to incorporate the processed galactomannan into the cell wall.