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. 2019 Feb 18;29(5):379–390. doi: 10.1038/s41422-019-0145-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — SGIP1 mediates thermomemory degradation of SGS3 and tasiRNA suppression. a Immunodetection of SGS3 abundance in 24-day-old plants. Coomassie blue staining of Rubisco served as a loading control. b Co-IP assays of SGIP1 with SGS3 in N. benthamiana leaves. Proteins were immunoprecipitated with an anti-FLAG antibody, and detected by western blot using the anti-SGS3 and anti-FLAG antibodies. c In vitro ubiquitination of SGS3 by SGIP1. Polyubiquitination of SGS3 by SGIP1 was detected by immunoblotting using the anti-SGS3 antibody. The mutated SGIP1 protein (SGIP1^△Fbox) served as a negative control. d SGIP1 promotes SGS3 polyubiquitination and degradation in vivo. Co-expression of SGIP1 and SGS3 was performed in N. benthamiana leaves. Protein accumulation of SGS3 and FLAG-SGIP1 was immunodetected with the anti-SGS3 and anti-FLAG antibody, respectively. GFP was co-expressed as an internal control. e Expression levels of SGIP1 as normalized to the ACTIN2 signals. Error bars indicate the SD (n = 3). Asterisks indicate significant difference between lines and 22 °C-grown Col (Student’s t-test; *P < 0.05, **P < 0.01). f Immunodetection of SGIP1 levels with an anti-SGIP1 antibody. ACTIN served as a loading control (d, f). g Knockdown of SGIP1 maintained the production of siR255 and siR1511 at 30 °C. U6 was used as a loading control. The intensity of the blots was quantified (a, f, g)