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. 2019 Feb 18;29(5):379–390. doi: 10.1038/s41422-019-0145-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — HTT5 coordinates thermomemory phenotypes via reduced tasiRNAs and the REF6-HSFA2 loop. a ChIP-qPCR validation of HSFA2 (left), REF6 (middle) and BRM (right) occupancy at the HTT5 loci. The analyzed regions were marked by bars. b HTT5 transcript levels in 24-day-old Col, hsfa2, ref6–1, brm-1 and ref6–1brm-1 grown at 22 °C and 30 °C. c Heat-induced upregulation of HTT5 could be transmitted to progeny, as detected by qRT-PCR. d Flowering times of indicated lines were assessed (n ≥ 15 for each line). e Disease symptoms at 4 dpi with Pst DC3000 (avrRpt2). Scale bar, 1 cm. f Bacterial growth was measured. Data are shown as means ± SD from three replicates (a, b, c, f). Lowercase letters indicate statistical significance based on one-way (a, c, d) or two-way (b, f) ANOVA with Tukey’s HSD post hoc analysis (P < 0.05)