Fig. 2.

Maturation of hHPLCs in vivo. a Spliced immunofluorescence staining of human ALB in hHPLC-engrafted URG mouse liver. The scale bars represent 2 mm. b The repopulation rate of hHPLCs in four mice was determined by ALB immunostaining 6 weeks after transplantation. c Human ALB secretion at 6 weeks post-transplantation from mice transplanted with hHPLCs (n = 9) and F-PHHs (n = 5). d Immunofluorescence staining of human ALB co-expression with human functional hepatic genes (CYP3A4, CYP2E1, CYP2D6, CYP2C9, CYP1A2, CYP2C8, CYP2C19, NTCP and MRP2) in hHPLC-engrafted URG mouse liver. For all measurements, ‘n’ represents the number of biological replicates. The scale bars represent 50 μm. Data are presented as mean ± SEM