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. 2019 Jun 18;29(8):641–654. doi: 10.1038/s41422-019-0193-0

Fig. 2.

Fig. 2

LncLrrc55-AS enhances IFN-I production in response to viral infection. a RT-qPCR analysis of lncLrrc55-AS silencing efficiency by two siRNAs (#1, #2). NC, a non-targeting control siRNA. b Fluorescence analysis of VSV replication in NC- or lncLrrc55-AS-silenced peritoneal macrophages infected with VSV-GFP for the indicated hours. c Determination of virus loads by TCID50 assay of the supernatant from macrophages infected with VSV for the indicated hours. d ELISA of IFNα and IFNβ in supernatant of control or lncLrrc55-AS-silenced macrophages infected with VSV for 12 h. e RT-qPCR analysis of lncLrrc55-AS or Ifnb mRNA expression in the selected WT or lncLrrc55-AS KO NIH/3T3 cell clones infected with VSV for 12 h. f RT-qPCR analysis of Ifna4 and Ifnb mRNA expression in empty vector control (EV Ctrl) or lncLrrc55-AS transfected lncLrrc55-AS KO NIH/3T3 cells infected with VSV for 12 h. Data are from three independent experiments (a, b right, c–f, means ± SEM) or are representative of three independent experiments with similar results (b left). *P < 0.05, **P < 0.01 and ***P < 0.005; ns, no significance (Student’s t-test or ANOVA)