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. 2019 Aug 12;44(8):615–630. doi: 10.1093/chemse/bjz050

Figure 5.

Figure 5.

Silencing NRSF/REST results in decreased differentiation of Odora cells in vitro. (A) REST mRNA levels in differentiated Odora cells, Odora cells treated with siNEG oligo (negative control), siGAPDH oligo (positive control), and a cocktail of 3 different siREST oligos. Gene expression levels were measured by RT-qPCR. Relative expression levels are shown as the ratio between REST expression and the housekeeping gene EF1a. ***P < 0.001. (B) OMP mRNA levels; (C) oCNGb mRNA levels; and (D) ADCY3 mRNA levels in undifferentiated Odora cells, differentiated Odora cells (Diff. positive control), Odora cells differentiated in the presence of S. aureus and the negative control silencing probe (siNEG), Odora cells differentiated in the presence of S. aureus and the GAPDH silencing probe, differentiated Odora cells and siREST probe (Diff. positive control + siREST), Odora cells differentiated in the presence of S. aureus and the siREST probe. Different letters denote statistically significant differences among treatments following 1-way ANOVA and Tukey’s post hoc test (P < 0.05).