Table 1. Mutagenic primers for CbuqPBP1.

Primers	Sequence
PBP1-F69A-Fm	5′-aatgcactattttctgtacagcgaaaaaattcgatttgatgaaag-3′
PBP1-F69A-Rm	5′-ctttcatcaaatcgaattttttcgctgtacagaaaatagtgcatt-3′
PBP1-H53A-Fm	5′-gatatccaagctctgatgaacgcggaacgaccagtcacccatgc-3′
PBP1-H53A-Rm	5′-gcatgggtgactggtcgttccgcgttcatcagagcttggatatc-3′
PBP1-F	5′-ggaattccatatgcttagcgaaagcttagttgttgatg-3′
PBP1-R	5′-ccgctcgagttaaaaactggtaattccaag-3′