TABLE 1.
E. coli strains and plasmids used in this study
| Strain or plasmid | Genotype or phenotype | Source |
|---|---|---|
| E. coli strains | ||
| DH10B pks+ | K-12 laboratory strain carrying pBACpks, Cmr | 1 |
| DH10B pks+ ΔspeB | speB mutant of strain DH10B pBACpks, Cmr Kanr | This study |
| DH10B pks+ ΔspeC | speC mutant of strain DH10B pBACpks, Cmr Kanr | This study |
| DH10B pks+ ΔspeE | speE mutant of strain DH10B pBACpks, Cmr Kanr | This study |
| DH10B pks+ ΔspeG | speG mutant of strain DH10B pBACpks, Cmr Kanr | This study |
| DH10B pks+ ΔspeE + p-speE | DH10B pks+ ΔspeE carrying p-speE, Cmr Kanr Ampr | This study |
| DH10B pks+ ΔclbA | clbA mutant of strain DH10B pBACpks, Cmr | 1 |
| DH10B pks+ ΔclbS | clbS mutant of strain DH10B pBACpks, Cmr | 13 |
| DH10B pks+ ΔspeE ΔclbS | speE clbS double mutant of strain DH10B pBACpks, Cmr | This study |
| M1/5 | Commensal E. coli strain isolated from feces of a healthy adult, B2 phylogenetic group, colibactin genotoxin producer |
35 |
| M1/5 ΔspeE | speE mutant of strain M1/5, Kanr | This study |
| SP15 | Extraintestinal pathogenic E. coli strain isolated from spinal fluid of neonate with meningitis, O18:K1 serotype, colibactin genotoxin producer |
50 |
| SP15 ΔspeE | speE mutant of strain SP15, Kanr | This study |
| SP15 ΔspeG | speG mutant of strain SP15, Kanr | This study |
| Plasmid | ||
| p-speE | pSC-A plasmid carrying wild-type speE gene, Ampr Kanr | This study |