TABLE 2.
Pilin Av frequencies of FA1090 strainsa
| Strain type | Pool A |
Pool B |
P value for A vs B |
Total |
P value vs FA1090 |
|||
|---|---|---|---|---|---|---|---|---|
| No. of reads |
% Av | No. of reads |
% Av | No. of reads |
% Av | |||
| FA1090 | 7,296 | 17.61 | 5,396 | 16.9 | 0.27 | 12,692 | 17.3 | NA |
| G4 mutant | 7,252 | 0.21 | ND | ND | NA | 7,252 | 0.21 | 2.4E−298 |
| FA1090 garP−10 | 4,707 | 0.1 | ND | ND | NA | 4,707 | 0.13 | 3.1E−200 |
| FA1090 garP−35 | 4,522 | 6.13 | 4,434 | 5.73 | 0.45 | 8,955 | 5.9 | 3.7E−135 |
a
Av frequencies of the FA1090 strain were calculated similarly to Table 1 using PacBio sequencing and analysis with SwitchAmp. P values were calculated by chi-square tests comparing Av read proportions between biological replicates (A versus B) or proportions of Av reads between summed totals of reads relative to the parental strain (FA1090). ND, not determined; NA, not applicable.