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. 2019 Oct 2;4(5):e00588-19. doi: 10.1128/mSphere.00588-19

FIG 9.

FIG 9

qRT-PCR showing miR-124-3p target gene expression in hNP cells upon JEV infection and miR mimic transfection. RNA isolated from control, control mimic-transfected plus JEV-infected, JEV-infected, miR-124-3p mimic-transfected, and miR-124-3p mimic-transfected plus JEV-infected hNP cells was subjected to qRT-PCR. JEV infection led to significant upregulation of PIM1, SDC4, PIK3CA, and IQGAP1 (upper panel) and CAV1, NRP1, SHC1, and MAP3K3 (lower panel) compared to control. However, upon mimic transfection and JEV infection, SDC4, PIK3CA, and IQGAP1 genes showed a reduced expression profile compared with that between JEV and JEV plus miR mimic groups. GAPDH gene expression was used as an internal control for normalization of PCR data. Data are representative of three independent experiments (mean ± SD) by one-way ANOVA (*, P < 0.05; **, P < 0.01; NS, not significant).