TABLE 4.
Effect of the blaOXA-837 gene from C. gilardii W2-2 on susceptibility to β-lactam antibiotics in the E. coli host strain
| Antibiotic | Diam (mm)a
|
|
|---|---|---|
| E. coli DH7298(pBAD18-cm) |
E. coli DH7299 (pBAD18-blaOXA-837) |
|
| Ampicillin | 26 | 20* |
| Amoxicillin-clavulanic acid | 24 | 23 |
| Cefotaxime | 40 | 39 |
| Ertapenem | 38 | 36 |
| Imipenem | 33 | 32 |
| Meropenem | 34 | 34 |
a
The diameter results shown are averages from five independent experiments. *, statistically significant differences (P < 0.002) between the E. coli strain with the empty pBAD18-cm plasmid (DH7298) and the strain with the codon-optimized blaOXA-837 gene from C. gilardii W2-2 cloned into pBAD18-cm (DH7299). Except for ampicillin, all other P values were >0.05.