FIG 8.

Deletion of C. gattii CDA3 in combination with any of the other two CDAs results in severe attenuation of virulence in CBA/J and C57BL/6 mouse models of infection. (A) CBA/J mice (6 to 8 weeks old, female) were infected intranasally with 10⁵ CFU of each strain. Survival of the animals was recorded as mortality of mice for 80 days postinfection (PI). Mice that lost 20% of the body weight at the time of inoculation were considered ill and sacrificed. Data are representative of two independent experiments with five animals for each strain. (B) C57BL/6 mice (4 to 6 weeks old, female) were infected intratracheally with 10⁴ CFU of each strain. Survival of the animals was recorded as mortality of mice for 50 days PI. Mice that lost 20% of the body weight at the time of inoculation were considered ill and sacrificed.