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. 2019 Aug 20;5:41. doi: 10.1038/s41421-019-0109-7

Fig. 2. Targeted exon skipping of CTNNA1 Exon 7 using ABE.

Fig. 2

a HEK293T cells were transfected with ABE 7.10 and a sgRNA targeting the splice acceptor site of CTNNA1 exon 7. Targeted exon skipping was observed after performing RT-PCR that could not be induced by the sgRNA alone, or in combination with dead Cas9 or D10A nickase Cas9. b Sanger sequencing of the shorter transcript confirmed exclusion of exon 7 from the CTNNA1 mature transcript. c High-throughput sequencing confirmed targeted A > G mutations in genomic DNA at the CTNNA1 exon 7 splice acceptor site