Open box represents open reading frame 1 (ORF1) which encoded a 2,105 amino acid (aa) polypeptide, a putative polyprotein around 242-kDa containing methyltransferase-like, papain-like protease, helicase-like, RdRp-like domains, and a coat protein (CP). ORF1 also contains variable region I (VRI) and variable region II (VRII). Open box with backslashes represents open reading frame 2 (ORF2) which is nested in open reading frame 1 and encoded a 36-kDa protein which belongs to 30-kDa superfamily of cell-to-cell movement protein (MP). Solid lines represent the 5' and 3' untranslated regions (UTRs). Short line with end points represent the citrus tatter leaf virus RT-qPCR detection assay targeting region designed in this study.