Table 11. Citrus tatter leaf virus RT-qPCR assay testing samples inoculated with non-targeted citrus pathogens.
Citrus Pathogen Isolate | Source / Registration number |
RT-qPCR Cq Value | ||
---|---|---|---|---|
COX | CTLV | Target | ||
Citrus tristeza virus (CTV) | ||||
T514 | T514-2 | 14.55 | - | 25.75 |
T538 | 3275–4 | 14.23 | - | 22.46 |
SY568 | 2761–114 | 13.87 | - | 20.30 |
Citrus psorosis virus (CPsV) | ||||
P201 | 1766–5 | 14.56 | - | 26.52 |
P203 | 2-26-98 | 14.93 | - | 28.38 |
P218 | 3175–2 | 14.06 | - | 28.67 |
Citrus leaf blotch virus (CLBV) | ||||
CLBV, Spain | 3069–1 | 14.86 | - | 27.89 |
Citrus vein enation virus (CVEV) | ||||
VE702 | 2923–2 | 14.46 | - | (+)* |
VE703 | 2923–3 | 14.33 | - | (+)* |
VE704 | 2923–4 | 14.42 | - | (+)*x |
Citrus yellow vein virus (CYVV) | ||||
YV3163-1 | 3163–1 | 14.54 | - | 21.28 |
YV3163-3 | 3163–3 | 15.09 | - | 20.72 |
YV920C | 3163–20 | 14.66 | - | 21.94 |
Infectious variegation virus (IVV) | ||||
IV400 | IV400 3-26-03 | 13.82 | - | 13.63 |
IV401 | IV401A 1993 | 14.62 | - | 28.41 |
Concave gum | ||||
CG302 | CG302 7-8-04 | 14.30 | - | (+)** |
CG308 | 2355–4 | 14.58 | - | (+)** |
CG309 | CG309 11-14-96 | 13.86 | - | (+)** |
Citrus viroids | ||||
Citrus exocortis viroid (CEVd) | 2765–1 | 14.43 | - | 26.17 |
Citrus bent leaf viroid | 2765–2 | 14.69 | - | 26.46 |
Citrus bent leaf viroid- LSS | 3237–3 | 17.05 | - | 32.76 |
Hop stunt viroid, non-cachaxia | 2765–4 | 14.92 | - | 21.62 |
Hop stunt viroid, cachaxia | 2765–6 | 17.39 | - | 27.34 |
Citrus dwarfing viroid | 2765–12 | 14.88 | - | 27.92 |
Citrus bark cracking viroid | 3200–1 | 15.13 | - | 23.45 |
Citrus viroid V | 3195–5 | 13.54 | - | 26.32 |
Candidatus Liberibacter | ||||
asiaticus | HLB B | 17.49 | - | 26.69 |
asiaticus | HLB G | 16.78 | - | 29.55 |
Spiroplasma citri | ||||
C189 | C189 7-8-09 | 16.95 | - | 30.11 |
S616 | S600 7-8-09 | 17.09 | - | 29.26 |
Abbreviations: Cq: quantitative cycle.
*Citrus vein enation virus was tested by conventional RT-PCR
**Concave gum was tested by biological indexing