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. 2019 Oct 17;14(10):e0224028. doi: 10.1371/journal.pone.0224028

Fig 1.

Fig 1

(i) Propidium iodide (PI) uptake showing death of gastric and colorectal cancer cell lines following a 4-hour melittin treatment. Cells were treated with 0.5–20 μg/mL melittin and the positive control was treated with 0.1% Triton X-100. Data shown as mean ± SD where n = 4. *** represents p = <0.001. EC50 values are 14 μg/mL for AGS, 11 μg/mL for COLO205 and 13 μg/mL for HCT-15. (ii) Florescence images showing Live/Dead staining of gastric and colorectal cancer cell lines following a 4-hour melittin treatment. A) AGS, B) COLO205 and C) HCT-15 cells were grown on poly-D-lysine coated coverslips overnight and treated with melittin at 5 μg/mL, 10 μg/mL and 20 μg/mL for 4 hours. The positive control was treated with Triton X-100. All cells were stained with Calcein-AM live stain (green) and EthD-1 dead stain (red). Average viability was determined for AGS (78% for 5 μg/mL, 17% for 10 μg/mL and 0% for 20 μg/mL), COLO205 (86% for 5 μg/mL, 40% for 10 μg/mL and 0% for 20 μg/mL), and HCT-15 (73% for 5 μg/mL, 50% for 10 μg/mL and 4% for 20 μg/mL). Viability for all negative and positive controls was 99–100% and 0% respectively. Scale bars represent 100 microns.