Fig 8. More lipophilic steroids preferentially accumulate in membrane fractions of cells.
(A) Fraction of the total steroid content in the membrane plus cytosol fractions that was contained in the membrane fraction for four different steroids after fractionation of LNCaP cells that were treated with 1 μM unlabeled steroid. Graphs show mean ± SD from two independent experiments each performed in triplicate for each steroid. Pregnenolone content in membrane fractions was greater than any other steroid (Tukey’s multiple comparison test after one-way ANOVA, p < 0.001 for all three comparisons). (B) Graph of logs of the data from (A) vs. log Kow of the four steroids. Slope of trendline is different from zero (p < 0.0001).