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. 2019 Oct 7;17(10):e3000444. doi: 10.1371/journal.pbio.3000444

Fig 1. Male LTCFDN mice show increased hepatic lipid content while Ad-TCF7L2DN infection increases hepatic lipogenic gene expression.

Fig 1

(A–B) Hepatic TG and FFA contents in 2-week-old mice (n = 6 for WT and LTCFDN mice; gender of the mice was not determined). (C–D) Hepatic TG and FFA contents in 12-week-old male mice. (E–G) Serum TG, FFA, and cholesterol levels in 12-week-old male mice. (H) HE and ORO staining in adult (24 weeks) mouse liver sections. Scale bar, 100 μm. (I) Quantification of ORO staining in panel H. (J) HA-tagged TCF7L2DN detection in MPHs 48 hours after Ad-TCF7L2DN infection. (K–L) ChREBP (Mlxipl) and SREBP-1c (Srebf1) mRNA detection in Ad-TCF7L2DN infected MPHs. (M) ChREBP and SREBP-1c downstream genes mRNA levels in Ad-TCF7L2DN-infected MPH. The 5 genes include those that encode Acc1, Ehhadh, Fas, Me1, and Scd1. *p < 0.05; **p < 0.01; ***p < 0.001. Values represent mean ± SEM. For panels C–G, N ≥ 4. Panel H are representative images. For Panel I and K–M, N ≥ 3. Underlying numerical values can be found in S1 Data. Acc1, acetyl-coA carboxylase; Ad-TCF7L2DN, adenovirus that expresses TCF7L2DN; ChREBP, carbohydrate-responsive element-binding protein; Ehhadh, peroxisomal L-bifunctional enzyme; Fas, fatty acid synthase; FFA, free fatty acid; GFP, green fluorescence protein; HA, hemagglutinin tag; HE, hematoxylin–eosin; Me1, cytosolic malic enzyme 1; MPH, mouse primary hepatocyte; ORO, oil red O; Scd1, stearoyl-coA desaturase-1; SREBP-1c, sterol regulatory element-binding protein 1-c; TCF7L2DN, dominant-negative TCF7L2; TG, triglyceride; WT, wild-type.