Fig 3. pgcAG47A is a gain of function mutation.
(A) Representative pictures showing growth of WT, ΔglmR, ΔglmR pgcAG47S on MH and (B) Bar graph showing CEF sensitivity. pgcA and pgcAG47S were ectopically expressed at amyE in ΔglmR and ΔglmR ΔpgcA and tested for (C) growth on MH medium (representative growth curves are shown; N>3) and (D) CEF sensitivity by disk diffusion assay using 6 μg CEF per disk. Where indicated, 1 mM IPTG was added. Standard deviation (error bars) is based on at least three biological replicates. Three asterisks represent statistical significance with P <0.001 with Tukey test.