Fig 4. pgcA_G47S suppresses a glmR deletion mutation independent of UDP-Glc biosynthesis.

(A) Representative pictures (N>3) showing LB with 20 mM MgSO₄ plates for WT and ΔglmR transformation with gtaB::mls chromosomal DNA, (B) A representative agarose gel (N>3) electrophoresis image showing PCR amplification of ΔglmR with internal check primers. (C) CEF susceptibility assay done with 6 μg antibiotic and (D) representative growth curve on MH medium. Standard deviation (error bars) is based on at least three biological replicates. Three asterisks represent statistical significance with P <0.001 with Tukey test. (E) Growth study on MH agar plates. The pictures were taken after overnight incubation at 37⁰ C. 1 mM IPTG was added when required in all experiments.