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. 2019 Oct 17;14(10):e0224108. doi: 10.1371/journal.pone.0224108

Fig 3. Representative ARMS-PCR results from the screening in the beta-tubulin gene at codon 200 in A. lumbricoides.

Fig 3

Genomic DNA samples from individual eggs were subjected to ARMS-PCR amplification with the two sets of primers. Lanes indicated by odd numbers correspond to ARMS-PCR products obtained using the primer combination Fs200Al + Rc200Al to detect the fragment without the mutation (92 bp), and the even-numbered lanes correspond to ARMS-PCR products obtained using the primer combination Fc200Al + Rr200Al to detect the fragment with the mutation (181 bp). In lanes 1–6, synthesized controls were used (lanes 1 and 2: wild-type plasmid; lanes 3 and 4: mutated plasmid; lanes 5 and 6: wild-type and mutated plasmid mix. For each sample, the two reactions were analyzed side-by-side. Products marked in red represent mutated samples. The image shows an agarose gel (2.0%) that was stained with GelRed™ (Biotium, USA). MW: 100-bp molecular weight.