Skip to main content
. 2019 Oct 17;14(10):e0223842. doi: 10.1371/journal.pone.0223842

Fig 1. GMTH Knockdown inhibits miR combo-mediated reprogramming.

Fig 1

Neonatal cardiac fibroblasts were transfected with negmiR (control) or miR combo and siRNAs that target Gata4, Tbx5, Mef2C, and Hand2 (non-targeting siRNA used as a control). (A) Expression of cardiac commitment genes was assessed four days after transfection by qPCR. N = 5. *Comparisons between negmiR and miR combo in the control siRNA and GMTH siRNA groups: *** P<0.0001, **P<0.001, *P<0.05. †Comparisons between GMTH siRNA groups and negmiR + control siRNA group: †††P<0.001, ††P<0.01, †P<0.05. (B) Expression of cardiomyocyte mature marker genes was assessed fourteen days after transfection by qPCR. N = 3–5. *Comparisons between negmiR and miR combo in the control siRNA and GMTH siRNA groups: **P<0.001, *P<0.05, ns not significant.