Figure 3. Heterozygous Atmin loss increases proliferation of lung tumor cells and tumor grafts.

A) Loss-of-heterozygosity testing for Atmin gene. Left, genotyping analysis of Atmin alleles in lung tumor cells and tail DNA. Right, number of genomic Atmin DNA copies, measured by Q-PCR and normalised to the 36B4 gene.

B) Graph showing the difference in cell proliferation between Atmin^ΔL/+ KP and KP cells.

C) Graph showing the volume of xenograft tumors at the indicated timepoints after subcutaneous injection of Atmin^ΔL/+ KP and KP cells.

D) Representative RNAScope images, showing Atmin and Ppib (positive control) mRNA in Atmin^ΔL/+ KP- and KP-derived xenografts.