Figure 2.

Jarid1b regulated HaCaT cell differentiation induced by high calcium. A, Expression of Jarid1b upon shRNA‐mediated knockdown at the mRNA and protein levels. B, RT‐qPCR and C, Western blot analysis of the expression of keratinocyte differentiation markers (K10 and IVL) upon Jarid1b silencing (#P < 0.01 shRNA group vs control at each time point, Student's t test). D, K10 protein expression upon cumate (100 and 300 μg/mL)‐induced Jarid1b overexpression in high‐calcium culture. E, HaCaT cell proliferation in low‐ and high‐calcium media upon Jarid1b overexpression or (F) knockdown (#P < 0.01 Jarid1b overexpression or knockdown group vs control at each time point, Student's t test)