Figure 5.

miR‐640 inhibited β‐catenin and EP300 expression. A, The schematic diagram of miR‐640 targeted to CTNNB1 and EP300. B, 293T cells were transfected with the indicated plasmids and miR‐640 mimic or inhibitor. Then, relative luciferase activities were measured to verify that miR‐640 directly bound to CTNNB1 and EP300 3’UTR and promoted its degradation. C,D, The expression of β‐catenin and EP300 was detected by qPCR (C) and Western blot (D) in the TNF‐α‐/ IL‐1β‐stimulated NP cells with or without miR‐640 presence. One‐way ANOVA was used to evaluate the significant difference for these data. *P ˂ .05; **P ˂ .01; ***P ˂ .001; ****P ˂ .0001