Figure 6.

RND3 promotes GBM cell apoptosis. A, Protein expression of RND3 and cleaved caspase 3 after overexpression and knockdown of RND3 in U87 cells. B, Apoptosis rates in U87 cells after overexpression and knockdown of RND3. C, Immunofluorescence analysis of JC1, reflecting the mitochondrial membrane potential (ΔΨm), in U87 cells after overexpression and knockdown of RND3. D, Quantitative analysis of ΔΨm in U87 cells after overexpression and knockdown of RND3. E, Immunohistochemical staining of cleaved caspase 3 and TUNEL staining in nude mouse tumours from the indicated groups (n = 12 for each group). Scale bar represents 50 μm in C and 100 μm in E