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Intracellular and cell membrane-attached mCherry in CT26.WT cells transfected with pls-A (no RSV-F; top panel) or pls-C (RSV-F ectodomain: bottom panel). Imaging: phase, phase visualized cells; mCherry expression, fluorescent signal of mCherry in its native or fusion configuration; anti-mCherry, cell staining with anti-mCherry antibody conjugated to Alexa Fluor 488 (only surface protein can be visualized); NucBlue stains nuclei in viable cells.
