Figure 7.

Representative images of tumors that recurred in most of the immunized animals at the site of initial injection at day 64. All mice were injected with (5 × 10⁴) CT26.WT tumor cells expressing mCherry and Hcp1/TssM. The mCherry signal at the site of injection was no longer detectable by the live animal imager in any of the mice by 24 days and there were no visible signs of tumors. Tumors recurred at the initial injection site, however, in all of the mice except in most of those initially immunized with the combined vaccine vac-B/C. The left panel demonstrates the absence of detectable mCherry fluorescence within the tumor; the right panel shows visible tumors.