Figure 8.

Decreased, but detectable Hcp1/TssM/mCherry levels found in cells from recurrent tumors. All recovered cell populations from the 64 day tumors were analyzed by qPCR on RNA (A) and qPCR on genomic DNA (B) for the presence of Hcp1/TssM sequences; mCherry signal was determined in the same cells by flow cytometry. Hcp1/TssM RNA sequences were markedly reduced, but not completely abolished in the recovered cells compared to the originally injected cells. In contrast, the signal for genomic DNA was very similar in the recovered and the original (positive control) cells. (C) mCherry expression in the recovered, originally injected, and non-transfected (negative control) cells indicate a reduction in fluorescence in recovered cells. (D) Representative flow cytometry data showing the reduced mCherry fluorescence, but very tight mCherry distribution profile of all recovered cells regardless of what DNA vaccine was originally use. The color code is the same in all pictures.