FIGURE 10.

Quantitation of flow cytometric analyses of the permeability of 10× MBC rifampicin-equivalent of 5-FAM-RIF into the washed Mtb MLP and NRP stage 2 cells before and after bead beating, and after release from hypoxia into normoxia. (A) Time course of the increase of 5-FAM-RIF fluorescence in the MLP and NRP stage 2 cells, before and after bead beating and from 48 h after release from hypoxia into normoxia. (B) Quantitation of the extent of fluorescence in the MLP and NRP stage 2 cells, before and after bead beating. (C) Levels of 5-FAM-RIF fluorescence in the MLP, NRP stage 2 hypoxia cells and in the NRP stage 2 cells at 0 and 2 h after 48 h post-release from hypoxia into normoxia, upon incubation with 5-FAM-RIF for 0 min (at 48 h, immediately upon release from hypoxia into normoxia) and 120 min (at 120 min after 48 h post-release from hypoxia into normoxia). Statistical significance was calculated using Student’s t-test. ^∗p ≤ 0.05.