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. 2019 Oct 11;10:2339. doi: 10.3389/fmicb.2019.02339

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Copyright © 2019 Jakkala and Ajitkumar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Calcofluor White (CFW; polysaccharide specific) staining of Mtb NRP stage 2 and MLP cells. (A–C) Images of NRP stage 2 cell under: (A) DIC; (B) CFW fluorescence; (C) merged images. (D–F) Images of MLP cell under: (D) DIC; (E) CFW fluorescence; (F) merged images. (G–J) Cell surface charge analysis of Mtb MLP, NRP stage 1, and stage 2 cells by Zeta potential analyzer. Zeta potential value of: (G) MLP cells; (H) NRP stage 1 cells; (I) NRP stage 2 cells. (J) Comparative zeta potential values of the MLP, NRP stage 1, and stage 2 cells. Statistical significance was calculated using Student’s t-test. ^∗p ≤ 0.05; ^∗∗p ≤ 0.005.