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. 2019 Oct 11;10:2420. doi: 10.3389/fimmu.2019.02420

Figure 2.

Figure 2

Analysis of purified rTbCLP. (A) Anti-His Western blot of protein purified from medium after 24, 48 and 72 h of induction. High molecular weight glycoisoforms (55–70 kDa) and low molecular weight glycoisoforms (35–45 kDa) are marked with arrows. (B) SDS PAGE and (C) Anti-His Western blot after deglycosylation of rTbCLP with Endo H. Protein samples were incubated with Endo H enzyme (+) or without Endo H (–) in denaturing conditions. Deglycosylated full form rTbCLP (48 kDa) and the shorter isoforms (35–38 kDa) are marked with arrows.