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. 2019 Oct 11;10:2391. doi: 10.3389/fimmu.2019.02391

Figure 4.

Figure 4

Effect of T cell stimulation on release of microvesicles from peripheral blood mononuclear cells. Peripheral blood mononuclear cells from healthy donors (n = 7) were incubated for 24 h with the TLR9-agonist ODN2395 in presence or absence of anti-CD3 antibody (OKT3). Microvesicles (MVs) released into the culture supernatants were subsequently isolated by differential centrifugation. Isolated MVs were quantified and characterized with respect to expression of G3BP and dsDNA by flow cytometry. (A) Concentration of MVs expressing G3BP (white columns), dsDNA (gray columns), or both (black columns) after subtraction of concentrations observed in non-stimulated cultures. (B) The corresponding fold change, expressed as the ratio between G3BP-expressing MVs in supernatants from stimulated and non-stimulated cultures (NS). (C) The corresponding fold change in the dsDNA-expressing MV population. Columns and error bars represent median values and interquartile range. *P < 0.05. NS, non-stimulated.